It comes after more than 300 staff are in isolation because of a positive covid case recorded on Friday.
Both drivers and operational staff have been tested and are isolating, it’s predicted it will disrupt the service for most of the week.
The Department of Transport has issued a statement assuring they have plans in place to ensure the service still runs.
"V/Line's COVIDSafe plan has a number of general contingency plans for different types of service disruptions, allowing it to modify services and put alternate transport options in place quickly whenever services are disrupted, including due to a COVID-19 exposure.”
This comes after the state of Victoria recorded a record number of 473 cases in the past 24 hours.
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